Introduction
The EZBioscience® EZ-press Serum/Plasma RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA (including mRNA, miRNA, lncRNA, CircRNA, et.al.) from human and animal serum/plasma. The purified total RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, Northern blotting, nuclease protection assays, cDNA library preparation after poly (A)+ selection, RNA sequencing, and so on. Serum/plasma samples are first lysed and homogenized in a strong denaturant and phenol containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. After homogenization, Buffer A is mixed with the lysate and incubated for 3 minutes. And then the mixture is centrifuged for phase separation; the upper-layer supernatant is collected and mixed with ethanol. The liquid is then passed through the RNA binding Spin Column containing a silica-based membrane to which the RNA binds. Impurities are effectively removed by subsequent washing. The purified total RNA is then eluted with Elution Buffer and may be used in a variety of downstream applications.
Components
Components | EZB-RN2 (50 Preps) |
Lysis Buffer | 30 ml |
Buffer A | 6 ml |
Wash Buffer 1* | 8 ml |
Wash Buffer 2* | 8 ml |
Elution Buffer | 25 ml |
Spin Columns (with Collection Tubes) | 50 Preps |
*Before using for the first time, add 32 ml of 100% ethanol to the Wash Buffer 1, 32 ml of 100% ethanol to the Wash Buffer 2.
Storage
Store the Lysis Buffer and Buffer A at 2 ~ 8°C, protect from light. Store other components at room temperature (When using these buffers, be careful to avoid of contamination). Divide the Elution Buffer into small aliquots upon reception is suggested.
Experimental Procedure at a Glance
For detailed information, please check the product manual.
