The EZ-press Cell to cDNA Kit PLUS offers a fast and easy method to produce cDNA from cells in culture without RNA isolation. cDNAs generated by this kit are suitable for gene cloning and quantitative analysis of gene expression, so it is a good substitution for the TRIzol method.
This kit offers several significant advantages compared with TRIzol method:
1. Easy to use. Starting from cells in culture, high quality cDNA can be synthesized in just two steps (cell lysis and reverse transcription), no RNA isolation is needed.
2. Fast. The whole experiment (from cells to cDNA) can be completed in less than 25 minutes.
3. Stable and highly reproducible. The total RNA is perfectly reserved throughout the experiment, enabling stable and highly reproducible results.
4. High sensitivity. High sensitivity. The lower detection limit for each sample is only 100 cells.
5. No Genome DNA left over. Genome DNA is removed even more adequately than the previous version. Moreover, the reagents used in this kit are safe and non-toxic, which is pleasant compared with the smelly and hazardous TRIzol reagent.
And Compared with EZ-press Cell to cDNA Kit, the template RNA can be collected from the cell lysate by ethanol precipitation, the dissolved in ddH2O. And then the RNA concentration can be detected by spectrophotometers, such as Nanodrop. So equal quantity of RNA from each sample could be employed as template in the following RT reactions. Further more, the EZ-press Cell to cDNA Kit PLUS has higher RT efficiency. The reverse transcription reactions carried out by this kit could be finished in only 15min.
Representative experimental results
